Synchrotron Based X-ray Microtomography Reveals Cellular Morphological Features of Developing Wheat Grain

Wheat is one of the most important crops in world, mainly used for human consumption and animal feed. To overcome increasing demand wheat production, it necessary to better understand mechanisms involved growth grain. X-ray computed tomography an efficient method non-destructive investigation 3D architecture biological specimens, which does not require staining, sectioning, or inclusion. In particular, phase-contrast results images with contrast increased resolution compared that obtained laboratory devices. The aim this study was investigate potential anatomy grain at early stages development. We provided entire grains various development stages. image analysis allowed identifying a large number tissues, visualize individual cells. Using high-resolution setup, finer details were obtained, making possible identify additional tissues. Three-dimensional rendering also revealed pattern resulting from epidermis appears as promising imaging plant organs